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Questions tagged [enzyme-kinetics]

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Inactivation of enzyme: Why inactivation constants do not follow the Arrhenius equation? [closed]

The Arrhenius equation may not account for the temperature effect on thermal inactivation rate constants. Why can the thermal inactivation kinetics not be expected to follow Arrhenius? The reason that ...
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High concentration of ferric ion catalyst decreases rate of decomposition of hydrogen peroxide in the Fenton reaction

I did a project on the Fenton reaction and rate of decomposition of $\ce{H2O2}$. I used colorimetry to measure the rate of decolourisation of methylene blue when I mixed $\pu{5e-6 mol}$ $\ce{FeCl3}$ ...
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Why enzyme-catalyzed reactions mostly go one way?

I listened to the lecture about enzymes. Professor downplayed the question in the subject. He said that enzymes are able to work both ways, but practically speaking reactions with overwhelming ...
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How to calculate the kinetic order of an enzymatic reaction?

This question is concerning the metabolism of ethanol by an alcohol dehydrogenase enzyme. Usually people metabolize alcohol equivalent to "one beer per hour". One beer is said to contain 33cL. The ...
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Are enzymes with superior catalytic properties more sensitive to temperature changes?

I am wondering whether enzymes with superior catalytic properties are more or less temperature sensitive than average enzymes. I know that enzymes lower the activation energy, Ea. So the more ...
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Enzyme Kinetics two enzymes in complex with one substrate

Given an enzyme reaction where two enzymes are required to form the complex, for example $\ce{2E +S-> E3S->2E +P}$, how would I calculate complex concentration assuming steady state ...
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Lowest Michaelis constant Km

I want to find a lower limit of the Michaelis constant for some evaluations of Michaelis-Menten enzyme kinetics. What is the lowest $K_m$ you ever encountered? Is there a theoretical limit?
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Michaelis-Menten rate law for enzyme which catalyzes two reactions: steady state?

Suppose an enzyme $\ce{E}$ can catalyze two reactions: \begin{align} \ce{S1 + E &<=> S1E -> P1 + E} \tag{R1} \\ \ce{S2 + E &<=> S2E -> P2 + E} \tag{R2} \end{align} I want ...
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Equations Used in Isothermal Titration Calorimetry

I have been attempting to understand and derive the formulas used for ITC which can be found here starting on page 310. I am particularly interested in the single set of identical sites. To first ...