Questions tagged [enzyme-kinetics]

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Why neglecting Bradford Solution Volume in the assay?

I would like to know why do we not need to take account of Bradford agent solution in dilution calculation? For example, $\pu{2.5 \mu g mL-1}$ BSA will calculate from $\pu{0.4 \mu L}$ of $\pu{1 mg mL-...
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Why does allosteric binding produce a sigmoidal curve?

Allosteric binding is where the enzyme can be regulated through having ligands bind onto somewhere that is not the active site. This will then induce a conformational change on the active site, hence ...
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Equations Used in Isothermal Titration Calorimetry

I have been attempting to understand and derive the formulas used for ITC which can be found here starting on page 310. I am particularly interested in the single set of identical sites. To first ...
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How to find the reaction rate in an enzyme reaction?

First time posting questions here, so let me know if I need to edit anything. I need to decide $dC/dt$ and $dP/dt$ when the reaction is like this, where I only know we must have 2 substrates $S$ to ...
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43 views

Solving for Concentration Using pseudo-First Order Kinetics

The following graphs represent a pseudo-first order bi-molecular reversible reaction with the formula $\ce{A + B <=> C}$: The reaction product has an extinction coefficient of $\pu{50000 M-1 cm-...
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Given the pH optimum of Angiotensin 1, how do I determine its charge?

I'm given the following information I'm then asked to determine the charge of the substrate Angiotensin 1 at the pH-optimum. I have found the pH-optimum to be 6.9. How do I go about answering this ...