I'm given the following information enter image description here

I'm then asked to determine the charge of the substrate Angiotensin 1 at the pH-optimum. I have found the pH-optimum to be 6.9.

How do I go about answering this question only knowing the optimum pH and the protein chain that Angiotensin 1 is composed of?

  • $\begingroup$ The only charged groups are the N-terminus, the C-terminus and the arginine side chain. Depending on conditions, there is a slight chance for the histitidine side chains to be charged as well. $\endgroup$ – Karsten Theis Dec 29 '19 at 21:39

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