From what I understand about the Michaelis Menten Model
- Km defines the amount of substrate required to reach half-saturation.
- 1/2 Vmax corresponds to Km on the x axis
- Generic formula is v = Kcat [ES] [S] / (Km + [S])
Given Km and the desired velocity in terms of reaction efficiency, can one just plug in Km into the equation and get a relational expression with [S] on one side?
Or do you manipulate the 1/2 Vmax expression?