I began working in a newly setup lab, and I'm having a dilysine (K2) peptide synthesized by solid phase method. I plan to cleave the peptides from the resin with a cleavage cocktail of TFA. Unfortunately, our freeze-drier is missing a part and not working then. We also don't have a rotary evaporator yet. We have our HPLC setup, but do you think it is a good idea to take the resulting solution of cleavage (after filtration to remove beads), which contains Boc, Fmoc, TFA, and possibly other impurities, directly to the HPLC?
I've read that HPLC sample should be dissolved in initial composition of my HPLC solvents, but it is in the case of having a crude dry sample.
It is my first time doing a peptide synthesis so I appreciate your ideas.