I would like to purify my peptide having cysteine residue by using HPLC. It is known that thiol containing peptides undergo oxidation and form disulfide linkages. In order to prevent it I used proper cocktail solution during cleavage from the resin. However, I am still worried what if my peptide undergo oxidation during HPLC purification? How can I prevent it?
I searched in research gate, but I couldn't find suitable answers.