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I would like to purify my peptide having cysteine residue by using HPLC. It is known that thiol containing peptides undergo oxidation and form disulfide linkages. In order to prevent it I used proper cocktail solution during cleavage from the resin. However, I am still worried what if my peptide undergo oxidation during HPLC purification? How can I prevent it?

I searched in research gate, but I couldn't find suitable answers.

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  • $\begingroup$ One way is to add reducing agent to the running buffer. I don't know of another way. $\endgroup$ – Buck Thorn May 20 '19 at 9:05
  • $\begingroup$ What reducing agent I can use? Any experience? $\endgroup$ – Science123 May 20 '19 at 9:26
  • $\begingroup$ This might be useful as a comparison: agscientific.com/blog/2019/04/… Also, I recommend searching a little more, it's not an uncommon problem. $\endgroup$ – Buck Thorn May 20 '19 at 9:33

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