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For part b(i) why is the spot tyrosine? I know tyrosine has an overall -2 charge while phenylalanine has an overall -1 charge. However, phenylalanine has a smaller Mr so shouldn't it travel faster? Also, I don't understand (ii)... why are they close?

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    $\begingroup$ So you figured out that the mobility depends on charge and on size. Make a table of the three species and their charge and size, and see if you can predict which travels the fastest. $\endgroup$
    – Karsten ♦
    Commented Aug 9, 2021 at 18:14

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Species Charge at pH 12 relative size
Dipeptide -2 Large
Tyrosine -2 Small
Phenylalanine -1 Small
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