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This tag is for questions concerning biochemical methods (e.g. electrophoresis) or those concerning biochemical mechanisms or research. Do not use this tag if your question is merely about compounds often used in areas related to biochemistry or associated with these. These may fall under organic chemistry or the appropriate compound’s functional groups’ tags.
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Acidity of thiamine - how comes hydroxide anion with pK about 15 ionizes thiamine with pK ab...
In the ionized structure shown, which looks like an ylid, the carbanion is formed on an $\mathrm{sp^2}$ orbital and no resonance stabilization of this charge is possible.
Regarding your question about …