I am a beginner chemistry student studying anticoagulant rodenticides. The literature shows that high-performance liquid chromatography (HPLC) with fluorescence or UV light is the most commonly used method for the detection of residues in tissue samples. I want to understand on a basic, conceptual level why this particular analytical method is the preferred method.
I understand that HPLC can separate complex organic compounds and enable detection at extremely low concentrations, but I do not know whether there are other analytical methods that can also do this.
Is this particular analytical method more effective in detecting the presence of residues from anticoagulant rodenticide in tissue samples? If so, why? Is the answer related to the chemical properties of the compounds in rodenticides (hydroxycoumarins and indandiones)?