I am making a stock solution of Tween 80 (0.05 % w/v) and each experiment requires just 15 ml of the stock solution. Is it possible to make a large batch (about 100 ml) and store it somewhere? If so, what should I look out for when storage?
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1$\begingroup$ Please note that nonconforming units such as "% w/v" should not be used in science and technology. $\endgroup$– LoongCommented Aug 16, 2022 at 17:16
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1 Answer
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The Sigma Aldrich/Merck product information for "TWEEN® 80 solution" states the following:
Storage/Stability
Aqueous solutions of polysorbates as well as the neat liquid will undergo autoxidation over time, with changes being catalyzed by light, increased temperature, and copper sulfate.[9]
Solutions are reasonably stable at 2 - 8 °C for short periods. For special applications, storage under argon or nitrogen may be preferred.
[9.] Donbrow, M., et al., Autoxidation of polysorbates. J. Pharm. Sci., 67, 1676-1681 (1978).
While this is not a very specific time span, it clearly indicates that TWEEN solutions are not long-term stable. If you need to store it, then in a fridge, protected from light and preferentially under an inert atmosphere for a few days max.
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6$\begingroup$ If you do this be sure to leave the date the solution was prepared on the bottle. $\endgroup$ Commented Aug 15, 2022 at 8:59