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Problem description

I have a substance that is extremely non-polar and therefore does not ionize in the ESI source, which makes it impossible to quantify small amounts. The substance contains only two functional groups, which are a point of attack for possible derivatization. One is an alkyne, and the other is a hydroxyl group. Both groups are attached to the same carbon. Since the substance is to be detected from blood plasma, I decided against derivatization at the hydroxyl group, since numerous side reactions with proteins, which also carry a lot of hydroxyl groups, are to be expected. The alkyne, however, is a good point of attack and is suitable for bioorthogonal reactions. A professor once said about the copper(I) catalyzed alkyne-azide cycloaddition (CuAAC) that this is a reaction that "any fool could manage". I have been trying to get the reaction going for a few days now, but so far, I have not been able to detect any product in LC-MS... Decide for yourself what this says about me as a chemist.

Since I have followed several approaches from papers, I slowly ask myself if my substance is not suitable for a CuAAC. Since the research project and the substance are new and unpublished, I cannot provide a complete structural formula here. But this much can be revealed: It is essentially a steroid, which carries a hydroxy group and an alkyne on carbon C17.

Substance before and after the CuAAC reaction

What was done

I performed the reaction with the pure substance (i.e. without blood plasma) according to Presolski et al., but I did not use THPTA nor aminoguanidine. All concentrations were used as described in the paper and the reaction was performed at 40°C according to the suggestion under "Troubleshooting". The azide was dissolved in DMF. Thus, there were 10 µL DMF (5%) in the buffer solution.

I also performed the reaction with the pure substance according to Hong et al.. Again, the proposed concentrations were used but neither THPA nor aminoguanidine were used.

I also tested different variations of those protocols with much longer reaction times (8 to 24 hours), more or less temperature, with and without pH 7 buffer.

As far as I know, the 4-substituted 1,2,3-triazoles formed by CuAAC should be extremely stable, even to metabolic degradation (Agalave et al.), so I do not believe that they decomposed so quickly that they were all destroyed by the measurement. To avoid ion suppression, I purified the reaction after the one-hour reaction time by RP-SPE, using the SigmaAldrich/Merck protocol.

Questions

  • Does anyone have experience with CuAAC reactions that do not want to run?
  • Which parameters should I at best adjust to make the reaction successful?
  • Is it possible that a hydroxide in alpha-position disturbs the reaction? I haven't found anything on this yet.
  • What advice is there to make the reaction successful even at very low (< 10 pg/mL) substance concentrations?

Update as of 24/07/2020

The reaction was, in fact, successful when applying the already cited protocol by Presolski et al. and using a DMF-water-ratio of 3:1 and incubating at 60 °C for 2 h. Unfortunately, though, the intensities are pretty weak which means that the reaction turnover is not great. The peak of my original substance is at 9.6 × 107 (50 µM concentration) and the intensity of the derivatized product, which should be way better ionized, is 4.2 × 107.

New question

  • How to get a better turnover while maintaining a reasonably short reaction time?
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  • $\begingroup$ Interesting question. I once got RuAAC to work when derivatizing internal alkynes for LC-MS, and CuAAC should be easier than RuAAC, since the Ru catalysts are very unstable to air/water. I'd recommend trying a Cu-chelating azide like picolyl azide, which should be more reactive. $\endgroup$ – Curt F. Aug 26 at 21:05
  • $\begingroup$ I'd also get a test alkyne that you can run your CuAAC protocol on. If you can make test alkyne-derived triazoles, then the problem is with your molecule. If you can't, then the problem is with your protocol (at least). $\endgroup$ – Curt F. Aug 26 at 21:06
  • $\begingroup$ I have no idea what "R" is in your structure or how the 5/6 rings are fused together or what the relative stereochemistry is at the carbon with the alkyne/alcohol. There may be an issue where the alkyne is in the "pocket" of the 5/6 fusion, and that steric bulk is really slowing the reaction. $\endgroup$ – Zhe Sep 10 at 16:44
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How to get a better turnover while maintaining a reasonably short reaction time?

To speed up the reaction, you should look at changing (1) the reagents, (2) the catalysts, or (3) the reaction conditions. In particular you might be dealing with poor solubility for your alkyne substrate if you are doing the reaction in aqueous conditions.

1. Reagents

You could try the picolyl azides I mentioned in the comments in lieu of the 4-acetophenyl azide you originally tried. These azide reagents chelate copper significantly, thus increasing reaction rates since proximity of two of the three components of the reaction (\ce{Cu+} and aizde) is enhanced by the chelation effect.

2. Catalyst

There are a variety of exotic $\ce{Cu+}$ organometallic complexes that work better than the "standard" copper salts. An example publication is Sebest et al. 2018, which reports that the N-heterocyclic carbene complex $\ce{[CuI(Mes‐6)]}$ (Mes-6 being an NHC ligand pictured below) worked well at loadings down to 0.05 mol% in their trial reaction.

Mes-6 NHC ligand

This might be overkill if you are not using the ligand THPTA used in your original protocol. Maybe you could just use that?

3. Conditions

You an always heat more, but I think a more important variable is your solvent. Is your reaction solvent one that dissolves all the species, including alkyne, azide, and copper(I) complex? Instead of aqueous conditions, can you try acetonitrile or 100% DMF? Or n-butanol?

Additionally, the Presolski reference you give mentions that high chloride concentrations (~0.2 mol/L) can compete with ligand for copper ion. Plasma has about a 0.1 mol/L chloride concentration, so maybe it's an issue? Probably speaks to the need to use a good copper ligand like THPTA.

Bottom line

If it were me, I'd try getting some THPTA or other ligand to improve $\ce{Cu+}$ solubility in organic solvents, and trying the reaction in non-aqueous solvents where your steroid is likely to be soluble. I'd also get the picolyl azide if my lab could afford it. If that doesn't work, and I still wanted to improve things, I guess I'd look at increasing temperature or using more exotic NHC ligands for the copper.

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