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Added tags; proper prefixes for micro-; redefined title
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andselisk ♦
andselisk ♦
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Optical density, Is Beer-Lambert law also valid for fluorescence, and luminescence?

When I measure optical density in a microplate reader the absorbance is proportional to the concentration and the height of the liquid. Is this law (Beer-Lambert Beer's law) also valid for fluorescence and luminescence? 

Let's say I prepare a fluorescent solution and measure e.g. the signal of 100uL100 μL and of 50uL50 μL. Do I get the half signal for 50uL50 μL?

Optical density, fluorescence, luminescence

When I measure optical density in a microplate reader the absorbance is proportional to the concentration and the height of the liquid. Is this law (Lambert Beer's law) also valid for fluorescence and luminescence? Let's say I prepare a fluorescent solution and measure e.g. the signal of 100uL and of 50uL. Do I get the half signal for 50uL?

Is Beer-Lambert law also valid for fluorescence and luminescence?

When I measure optical density in a microplate reader the absorbance is proportional to the concentration and the height of the liquid. Is Beer-Lambert law also valid for fluorescence and luminescence? 

Let's say I prepare a fluorescent solution and measure e.g. the signal of 100 μL and of 50 μL. Do I get the half signal for 50 μL?

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cekar
cekar
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Optical density, fluorescence, luminescence

When I measure optical density in a microplate reader the absorbance is proportional to the concentration and the height of the liquid. Is this law (Lambert Beer's law) also valid for fluorescence and luminescence? Let's say I prepare a fluorescent solution and measure e.g. the signal of 100uL and of 50uL. Do I get the half signal for 50uL?