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andselisk ♦
andselisk ♦
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This answer will change fairly frequently because technologies are always improving. These days, I think a bare minimum list might be something like:

  1. a 2.1 mm × 50 mm C18 column for rapid reversed-phase methods.
  2. a 2.1 mm × 150 mm C18 column for slower, broader-coverage reversed phase methods.
  3. a 2.1 mm × 50 mm HILIC column for rapid methods to detect polar metabolites.
  4. a 2.1 mm &times× 150 mm HILIC column for slower, broader-coverage methods to detect polar metabolites.

Some further notes:

  • If your lab is going to use ion-pairing based methods, you will probably want separate columns for ion-pairing methods and "clean" methods without ion-pairing reagent. Ion-pairing agents like tributylamine and/or perfluoroheptanoic acid are very difficult/impossible to remove from columns once they have been used for this purpose.

  • Fairly soon the best answer might be to use 1.0 mm diameter columns instead of the "standard" 2.1 mm diameter that is most common nowadays.

  • The most widely used HILIC column seems to the "BEH Amide" column chemistry from Waters. Following close behind may be ZIC-HILIC from SeQuant and/or Agilent's HILIC-Z chemistry.

  • For C18 chromatography, performance differences between different vendors are comparatively minor (although real). A wide variety of chemistries are used.

This answer will change fairly frequently because technologies are always improving. These days, I think a bare minimum list might be something like:

  1. a 2.1 mm × 50 mm C18 column for rapid reversed-phase methods.
  2. a 2.1 mm × 150 mm C18 column for slower, broader-coverage reversed phase methods.
  3. a 2.1 mm × 50 mm HILIC column for rapid methods to detect polar metabolites.
  4. a 2.1 mm &times 150 mm HILIC column for slower, broader-coverage methods to detect polar metabolites.

Some further notes:

  • If your lab is going to use ion-pairing based methods, you will probably want separate columns for ion-pairing methods and "clean" methods without ion-pairing reagent. Ion-pairing agents like tributylamine and/or perfluoroheptanoic acid are very difficult/impossible to remove from columns once they have been used for this purpose.

  • Fairly soon the best answer might be to use 1.0 mm diameter columns instead of the "standard" 2.1 mm diameter that is most common nowadays.

  • The most widely used HILIC column seems to the "BEH Amide" column chemistry from Waters. Following close behind may be ZIC-HILIC from SeQuant and/or Agilent's HILIC-Z chemistry.

  • For C18 chromatography, performance differences between different vendors are comparatively minor (although real). A wide variety of chemistries are used.

This answer will change fairly frequently because technologies are always improving. These days, I think a bare minimum list might be something like:

  1. a 2.1 mm × 50 mm C18 column for rapid reversed-phase methods.
  2. a 2.1 mm × 150 mm C18 column for slower, broader-coverage reversed phase methods.
  3. a 2.1 mm × 50 mm HILIC column for rapid methods to detect polar metabolites.
  4. a 2.1 mm × 150 mm HILIC column for slower, broader-coverage methods to detect polar metabolites.

Some further notes:

  • If your lab is going to use ion-pairing based methods, you will probably want separate columns for ion-pairing methods and "clean" methods without ion-pairing reagent. Ion-pairing agents like tributylamine and/or perfluoroheptanoic acid are very difficult/impossible to remove from columns once they have been used for this purpose.

  • Fairly soon the best answer might be to use 1.0 mm diameter columns instead of the "standard" 2.1 mm diameter that is most common nowadays.

  • The most widely used HILIC column seems to the "BEH Amide" column chemistry from Waters. Following close behind may be ZIC-HILIC from SeQuant and/or Agilent's HILIC-Z chemistry.

  • For C18 chromatography, performance differences between different vendors are comparatively minor (although real). A wide variety of chemistries are used.

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Curt F.
Curt F.
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This answer will change fairly frequently because technologies are always improving. These days, I think a bare minimum list might be something like:

  1. a 2.1 mm × 50 mm C18 column for rapid reversed-phase methods.
  2. a 2.1 mm × 150 mm C18 column for slower, broader-coverage reversed phase methods.
  3. a 2.1 mm × 50 mm HILIC column for rapid methods to detect polar metabolites.
  4. a 2.1 mm &times 150 mm HILIC column for slower, broader-coverage methods to detect polar metabolites.

Some further notes:

  • If your lab is going to use ion-pairing based methods, you will probably want separate columns for ion-pairing methods and "clean" methods without ion-pairing reagent. Ion-pairing agents like tributylamine and/or perfluoroheptanoic acid are very difficult/impossible to remove from columns once they have been used for this purpose.

  • Fairly soon the best answer might be to use 1.0 mm diameter columns instead of the "standard" 2.1 mm diameter that is most common nowadays.

  • The most widely used HILIC column seems to the "BEH Amide" column chemistry from Waters. Following close behind may be ZIC-HILIC from SeQuant and/or Agilent's HILIC-Z chemistry.

  • For C18 chromatography, performance differences between different vendors are comparatively minor (although real). A wide variety of chemistries are used.