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I feel you have a misconception. ESI is an ionization technique not a detection technique. The sole purpose of the electrospray ionization is to impart a charge on a molecule in the gas phase. The molecule could be neutral, anionic or cationic in the solution phase. The inventor of ESI used to say that ESI makes the elephants fly, i.e., very large molecules ...


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Whenever an electron jumps from one orbit to another it either looses or gains energy in form of radiation. When energy is supplied to an atom the electron jumps from one orbit to its any one of the higher orbits. Now electron may jump directly to its final orbit or can jump to the next orbit and then the next till final orbit. So, whenever it jumps from one ...


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