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There are two possibilities: If there is only one optical pathway, you record i) the blank sample for all wavelengths of interest and store the detector's intensity information in function of $\lambda$ in a working memory. Light passing the blank is then assumed to equate $I_0$. Then you record ii) the wavelength dependent intensities $I_\mathrm{abs}$ with ...


4

Let me provide the intuitive idea so that you take the lead from here and understand the notion behind "instrument response" function. Any analytical/physical chemist, who uses an intrument, should have some basic idea of the instrument response. After that you should look up formal concepts of convolution on Youtube. Whenever you collect ...


2

Suppose that the lifetime of electronically excited atoms or molecules is to be measured by exciting them with a pulse of light and their fluorescence measured as it decays with time. This fluorescence could be observed with a photodiode or photomultiplier, whose output voltage is measured with an oscilloscope. Before doing this experiment, two questions ...


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