New answers tagged proteins
0
In this report, diaminopropane is used to elute a lectin from an affinity column. They mention the pH, which is the key to the question.
The bound lectin was eluted with 20 mM diaminopropane containing 0.15M NaCl, pH ~11.
The diaminopropane is unbuffered, and the high pH weakens the interactions between lectin and carbohydrates.
Top 50 recent answers are included
