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If the substrate still binds to the protein, other parts of the mechanism are still in place. Specifically, the main chain amide groups that help to stabilize the tetrahedral intermediate are still present. In the absence of serine, water will attack the carbonyl. In the presence of serine and the absence of the histidine, serine will still be the ...

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Short answer: under anaerobic conditions, it is impossible to produce acetate as the sole product from glycerol with wild-type E. coli. Explanation: Under anaerobic conditions (and assuming no substrates for anaerobic respiration have been added), a significant concern is redox balancing. Since electrons are not transferred to oxygen (as they are under ...

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A bit of a primer first: The body's sleep cycle is regulated by Melatonin, which is a hormone produced by the pineal gland. You can read more about this hormone here but in summary, for a biochemical solution to keeping someone awake, you would want to either catalyse the breakdown of melatonin in the body or inhibit melatonin secretion by the pineal gland. ...

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Based on the discussion in comments, the OP is interested in the equilibrium concentration of the species ABC in this reaction scheme: $$\ce{A + B + C <=>[k_1][k_2] AB + C <=>[k_3][k_4] ABC}$$ Equilibrium is reached when the rates of the forward and backward reactions of each step are equal. Thus, we have have two equations describing the ...

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