Questions tagged [biochemistry]

This tag is for questions concerning biochemical methods (e.g. electrophoresis) or those concerning biochemical mechanisms or research. Do not use this tag if your question is merely about compounds often used in areas related to biochemistry or associated with these. These may fall under organic chemistry or the appropriate compound’s functional groups’ tags.

919 questions
Filter by
Sorted by
Tagged with
33 views

What is name of this formulas? [closed]

i have a question what is name of this thing (it should be something with proteins) Thanks for all answers
43 views

Theoretically, given an ample supply of energy, can I convert Carbon Dioxide and Water into C6H12O6 and Oxygen in a Chamber? [closed]

Long story short, I have an insane volume of Carbon Dioxide and I have a bunch of experiments in mind. One of my questions is, would it be theoretically possible to release a high volume of Carbon ...
70 views

To which extent is protein folding conserving subprotein structures?

I am actually a pure mathematician, who stumbled over this paper «Protein-Folding Analysis Using Features Obtained by Persistent Homology» by Ichinomiya et al. (Biophys. J. 118, 2020, 2926-2937; link),...
10 views

Nanocomposite hydrogel for wet adehsion in biomedical applications

my name‘s Tristan and I‘m a bachelor‘s student in Germany. I‘m trying to synthesize an injectable nanocomposite hydrogel for wound closure and wound healing applications. The problem is that, next to ...
27 views

Gluconeogenesis

So I've been doing research on the production of lactic acid, however, I found that lactate will first reform into pyruvate and then go through gluconeogenesis reforming D-glucose. From what I ...
34 views

73 views

Carbon atoms types in amino-acids [closed]

I'm looking for a table or another kind of schema where I can find which type of carbon atoms (sp3, sp2 etc.) are present in each amino-acid. In my biochemistry books these informations are not ...
27 views

Do phosphoryl thiocholines, their uncharged analogs, and other organophosphates, interact differently with Acetylcholinesterase?

As asked in the title, would the various salts -such as hydrochloride or methyl iodide- of V-Agents interact any differently with acetylcholinesterase compared to the neutral compound? For instance, ...
44 views

What do we know about the structure of the amylopectin iodine complex?

I am studying a method of finding the ratio between amylose and amylopectin using the difference in color of the amylose-iodine complex(blue) and the amylopectin-iodine complex (red/brown). Through ...
26 views

What is meant by the term 'biosynthetic origin'?

I keep seeing the term biosynthetic origin used in medicinal related papers. Does it just refer to the biological precursors to the molecule? So, for example, the biosynthetic origin of Paclitaxel is ...
134 views

Why is the formation of a peptide bond not spontaneous?

Why is the formation of a peptide bond from a carboxylic acid and amine not spontaneous? I'm not looking for the free energy calculation; I have it from several sources. I'm just asking for an ...
2k views

Why do green bell peppers become bitter when cooked?

Why do green bell peppers taste sweet raw, but become bitter when cooked (steamed)? What chemical process is responsible for this? (I don't believe the temperatures are high enough for the Maillard ...
30 views

Why can't NAD+ undergo single-electron reduction?

$\ce{FAD}$ can undergo single-electron reduction to form a stable radical, which can then be reduced again to $\ce{FADH2}$. This is supposedly possible due to resonance stability, where the unpaired ...
54 views

How to run electrochemical tests on Microorganism (bacteria in a medium)?

I am trying to run some electrochemical tests(e.g.CV (Cyclic Voltammetry) & EIS (electrical impedance spectroscopy)) using half cell configuration on micro-organisms (e.g.bacteria). The bacteria ...
48 views

Why is the hydrolysis of ATP to AMP more energetic than the hydrolysis of ATP to ADP?

I am confused as to why the hydrolysis of ATP to AMP and pyrophosphate is more energetic than hydrolysis to ADP and inorganic phosphate, especially since pyrophosphates are more unstable than ...
17 views

How carbon nanotube sensors works?

How carbon nanotube sensors works? Is the protein (or chemical compound used as part of the sensor) placed inside the tube or where is it placed? and how is the technology used to place the protein ...
35 views

Michaelis–Menten kinetics applied to a solution with two enzymes

Two enzymes are added to a solution containing a tow concentration of a substrate that they can both use. Which statement is correct? A. Both enzymes will use equal amounts of the substrate. B. ...
103 views

How do enzymes affect a reaction's equilibrium?

I am not sure to understand something I read in a educational journal (1) Introduction Lets consider the following reversible enzyme-catalysed reversible reaction; E + S \leftrightharpoons ES \...
2k views

In sourdough bread baking what do the wild yeast and bacteria not eat during dough fermentation and why?

I bake bread using a sourdough starter. The starter is a culture of lactobacillus and some kind of yeast. They apparently eat the sugars or carbohydrates in the dough during fermentation but when I ...
101 views

Does anyone know what program was used to make this molecular structure picture? [closed]

I am a quantum chemistry beginer, and this picture color scheme is classic. I wonder how to make such pictures by myself.
59 views

How ph affects aesculune fluorescence?

I am facing a ween problem. I am trying to find out, how would look like the graph for fluroscence of aesculine (intensity of fluoroscence on Y axis and pH on X axis). I know, that increasing the pH ...
641 views

Can sugars dissolve in liquid ammonia?

Can monosaccharides and disaccharides dissolve in liquid ammonia due to hydrogen bond formation? My rationale is that these sugars may be able to form hydrogen bond with ammonia ($\ce{NH3}$).
91 views

Biological activity of the isotopes of the same element

I have a question regarding biological activity of different isotopes of the same elements, notably $\ce{^206Pb}$ and $\ce{^208Pb}$. Lead is known to be toxic, such that it can replace calcium from ...
32 views

Differences in AChE inhibition kinetics between paraoxon and methyl paraoxon

According to literature, dimethyl paraoxon has a reaction rate constant for the aging (spontaneous dealkylation) of acetylcholinesterase of $\pu{0.186 h-1}$, a spontaneous reactivation rate constant ...
67 views

Haworth structures of sucrose and lactose

The haworth structures of lactose and sucrose is My question is regarding the glyosidic linkage, in lactose it is represented as a zig zag and in sucrose it is a "V". What does that mean. ...
23 views

Differences in AChE inhibition kinetics between VX and VR

According to the referenced paper, VR has an AChE inhibition reaction rate constant almost 4 times that of VX. Interestingly, its reaction rate constant for aging of the enzyme is less than that of VX,...
382 views

Why does denaturation of albumen protein cause pH of solution to increase?

I'm interested in the denaturation of proteins by alcohols, specifically by ethanol. I have devised a simple experiment in which I inject solutions of ethanol (of variable concentrations) into ...
14 views

Structure-Activity Relationship of Chlormephos and Parathion

According to the book "The Chemistry of Organophosphorus Pesticides", the insecticide Chlormephos (S-(chloromethyl) O,O-diethyl phosphorodithioate) has an oral LD50 in rats of 7 mg/k. ...
28 views

Why is there a mirror image effect in the absorbance and fluorescence spectra?

I've read some answers online but I still can't seem to understand the mirror image effect. Why is the highest energy absorption (v" = 0 to v' = 4) the lowest energy fluorescence (v' = 4 to v&...
30 views

How can DI water inhibit peroxidase activity?

I'm troubleshooting an IHC staining issue, and according to Thermo-Fischer's website, "Deionized water can sometimes contain peroxidase inhibitors that can significantly impair enzyme activity.&...
29 views

Which electrons are in HOMO in a peptide bond?

We've recently studied the absorption of UV light by polypeptides, and found that the peptide bonds itself absorhs UV light at around 210nm. I'm confused about what feature causes this to happen.
87 views

How do point mutations affect complementary base-pairing and widths of the nucleotide pairs?

Point mutations are divided into two categories: transitions and transversions. Since point mutations only occur during DNA replication (an integral process of both mitosis and meiosis), the mutated ...