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Results tagged with Search options user 137

This tag is for questions concerning biochemical methods (e.g. electrophoresis) or those concerning biochemical mechanisms or research. Do not use this tag if your question is merely about compounds often used in areas related to biochemistry or associated with these. These may fall under organic chemistry or the appropriate compound’s functional groups’ tags.

3
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I did some bioconjugation with NHS and maleimide biotins to proteins, peptides, and oligosaccharides. I removed the free biotin using spin filters or dialysis. Just be sure to use a MWCO well below th …
answered Jul 27 '14 by user137
2
votes
There are mutants of clotting factor VIII that have longer half lives and better activity than natural Factor VIII. Factor VIII is one of the missing proteins commonly involved in hemophilia. Hemophil …
answered Jul 31 '14 by user137
6
votes
Since you're concerned about mercury from fillings, you may have already taken a look at the wikipedia article about dental amalgam toxicity. It would be worth reading through some of the sources list …
answered Aug 19 '14 by user137
2
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Our lab uses a thiazole orange exclusion assay to measure how well our polyintercalating peptides* bind DNA. The assay works by mixing thiazole orange with DNA so it becomes fluorescent. Then we measu …
answered Nov 5 '14 by user137
1
vote
High concentrations of ethanol might work well if you just want to put it on skin. Ethanol should be safe, it's the main ingredient in those hand sanitizers.
answered Aug 4 '14 by user137
3
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Most assays of carcinogenicity are designed to measure DNA damage. The 2 most common of those assays are the Ames test and the Comet Assay. The Ames test uses a special set of strains of bacteria tha …
answered Aug 21 '14 by user137
2
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If you're a little looser with your definition of monomer, then Tubulin fits right in. Alpha and Beta Tubulin proteins bind to each other to form Microtubules which are an important component of cell' …
answered Jan 8 '15 by user137
4
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2answers
I asked this in biology, but I think they don't want to deal with the chemistry behind UV-Vis. My question has to do with the Poly A tails on mRNA. I've been doing in vitro transcription and tailing …
asked Sep 5 '14 by user137
1
vote
I played around with a program called GROMACS about 4 years ago and don't remember much, but I do remember doing a tutorial simulating a DNA double helix and part of it included making the DNA by givi …
answered Jul 27 '14 by user137
2
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If all you want is a practical answer, just try taking a constant mass of dough, dissolve in a constant amount of deionized water, and measure the pH with a standard pH meter. The pH of the measured s …
answered Jul 31 '14 by user137