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Tagged with structural-biology chemical-biology
3 questions with no upvoted or accepted answers
6
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How to model CRISPR/Cas9 binding and cleavage using a generalized Kittel's zipper model?
I am thinking about how to model the binding of a CRISPR/Cas9+guide-RNA complex to a double-stranded DNA that cause its cleavage.
This post describes four steps at which a CRISPR/Cas9 system binds and ...
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4
votes
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answer
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Is it possible to have an anhydrous solvent for virology, specifically in vitro phage assembly?
Typical protocols for lambda phage in vitro assembly call for you to dump all your viral components and buffer solutions into water. Water is a great solvent, drives extraction of energy from atp, and ...
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Is DNA a base inside the cell?
DNA is deoxyribonucleic acid because it has phosphate groups in every one of it's nucleotides.
However, in aqueous solutions protons will ionize, leaving phosphates alone as negative groups, i.e., ...
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