Questions tagged [chromatography]

collective term for a set of laboratory techniques for the separation of mixtures

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4
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1answer
363 views

What inference can be made from the fact that there is no reversal in how three components eluate?

I gave an answer this question about TLC. I now realize that I am blundering my way through this question. The problem statement doesn't specify if the compounds to be eluted are polar or non-polar. ...
3
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1answer
2k views

How do I perform thin-layer chromatography to identify alkaloid content in fruits?

Fruits and as well as other plant parts usually contain alkaloids, which are nitrogenous compounds. I'd like to know how the Thin-Layer Chromatography method can be used to determine the alkaloid ...
2
votes
1answer
1k views

The Principle of Internal Standardization (in the context of ion chromatography)

I am trying to grasp how internal standardization works in principle, and I am having some trouble wrapping my head around it. It is not the choosing of an IS I think about, orwhen it might benefit ...
4
votes
1answer
958 views

How do I find Rf values & length size of paper in chromatography?

A student is designing an experiment using paper chromatography. From previous results, she knows that the Rf values for two dyes that she wishes to investigate are 0.72 and 0.80. It is proposed ...
3
votes
1answer
435 views

Diffusion/Streaky bands on TLC plate

These days I am working on a research project where I am supposed to run TLC for samples which are wax extracts from coconut butten nuts using chloroform as the solvent. All the samples have been ...
0
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2answers
14k views

How does the polarity of the eluent and sample affect the Rf value in thin layer chromatography?

A three-component mixture (X, Y, Z) was separated using normal phase TLC. Three eluents of varying polarity were used. Select the TLC plate that was developed using the least polar eluent (click on ...
0
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2answers
931 views

Retention time and dead time

Why does ts start just after tm ? Does the analyte starts spending time in the stationary phase only after the mobile phase has passed and reached the detector ? Wouldn't there be an overlap between ...