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lets say we increased the excitation wavelength higher than it should be, what effect would this have on the sensitivity on our analysis, or changing the emission wavelength to a higher value than it would be. My guess is that the higher wavelength leads to a lower frequency and so lowers the sensitivity but I have no idea what changing the emission wavelength would do?

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    $\begingroup$ How would you change the emission wavelength? $\endgroup$ – Klaus-Dieter Warzecha Apr 9 '14 at 8:01
  • $\begingroup$ sorry I mean if we were to raise the wavelength of the florescence spec used to excite the molecules. $\endgroup$ – Unistudent9 Apr 9 '14 at 9:45
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    $\begingroup$ @KlausWarzecha - You could also choose to monitor the emission in a single wavelength experiment at wavelengths $\ne \ \lambda_{max}^{em}$. You may want to do this, for example, to avoid matrix effects. $\endgroup$ – Ben Norris Apr 9 '14 at 10:24
  • $\begingroup$ @BenNorris Absolutely true! Excitation spectra measured at different wavelength plus repeated experiments after dilution aren't the worst idea. The world is full of dimers ;) $\endgroup$ – Klaus-Dieter Warzecha Apr 9 '14 at 11:29

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