# Fluorescence spectrocopy, sensitivity

lets say we increased the excitation wavelength higher than it should be, what effect would this have on the sensitivity on our analysis, or changing the emission wavelength to a higher value than it would be. My guess is that the higher wavelength leads to a lower frequency and so lowers the sensitivity but I have no idea what changing the emission wavelength would do?

• How would you change the emission wavelength? – Klaus-Dieter Warzecha Apr 9 '14 at 8:01
• sorry I mean if we were to raise the wavelength of the florescence spec used to excite the molecules. – Unistudent9 Apr 9 '14 at 9:45
• @KlausWarzecha - You could also choose to monitor the emission in a single wavelength experiment at wavelengths $\ne \ \lambda_{max}^{em}$. You may want to do this, for example, to avoid matrix effects. – Ben Norris Apr 9 '14 at 10:24
• @BenNorris Absolutely true! Excitation spectra measured at different wavelength plus repeated experiments after dilution aren't the worst idea. The world is full of dimers ;) – Klaus-Dieter Warzecha Apr 9 '14 at 11:29