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Would it be better to use water or ethanol as a solvent for syringaldazine? Why? Would water interfere with the oxidation of syringaldazine if laccase enzyme was added?

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  • $\begingroup$ I refer to a post, which refers to a paper: researchgate.net/post/… "The assay mixture contained 0.2 mM syringaldazine (dissolved in 60% ethanol) and 40 mM citrate-buffer, pH 5.6. Oxidation of syringaldazine was followed by an absorbance increase at 525 nm (ϵ525 = 65 000 dm3 M−1 cm−1) at a temperature of 25°C" Ignoring what is stated in the link (which confirms the idea) I doubt that water would interfere, since the molecule is stable in water, and since the enzyme works in a environment in which water is present $\endgroup$ – The_Vinz Apr 22 '18 at 15:13
  • $\begingroup$ The use of ethanol is necessary in order to dissolve the otherwise poorly soluble substrate (21.06 mg/L in water at 25°C) $\endgroup$ – The_Vinz Apr 22 '18 at 15:14

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