PEP (Phosphoenolpyruvic acid) is acidic but I need to make it neutral. I need a solution (pH 6-8) of PEP in a final 1 mL volume with a concentration of 100 mM.
How would I make this?
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For our work, we also need to make 100 mM PEP and we neutralize it using KOH. Traditionally, using Na+ based salts isn't ideal for biological assays so K+ salts are better for our applications.
Alternatively, if you're adding the PEP to a different solution, you can buffer the reaction solution. While the Phosphate does have a good amount of buffering capacity, effective concentrations of the molecule will be low so you ultimately won't be adding much.