I'm trying to work backwards to obtain the original concentration of a 1 litre solution. I feel my calculations have gone wrong and need another set of eyes to have a look.
To start, a tablet containing arginine was dissolved in water and made up to 1 litre solution. A 250 μL aliquot was taken from the solution and diluted to 25mL.
Analysis was done and a concentration value for the 25mL sample was 4.25 x10^-5 mol/L.
When I tried to work backwards from that concentration value and determine what the original concentration is in the 1 litre solution, I got 17 mol/L. Which seems excessively high for what was suppose to be a tablet.
I then need to give the value of mass of arginine (172.2 g/mol) in the tablet, but using the 17 mol/L, gives a value ~3kg.
What do I need to do to get the concentration value for the 1 litre solution?