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I need to maintain a certain range of % of ionisation from a buffer.

For e.g. Histidine buffer. When making high concentration of this buffer at (say) pH 7.2, 25'C, [concentration] is limited to 75mM solution (250mL), due to the solubility. Adding HCl increases solubility just as much, but it also decreases pH of the solution.

I want to know if there's a calculation or a formula to figure out the concentration limits (at least the highest concentration) of a particular buffer one can plan to make instead of trial and error in the lab?

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  • $\begingroup$ @Martin - マーチン even giving a bounty, I did not get an answer or response to my previous questions. I just wonder if there's anyway to receive community attention and support? $\endgroup$ – bonCodigo Jun 20 '17 at 5:31
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    $\begingroup$ bon, you can only ping someone in a comment if they're the author or an editor of that particular post. You would need to ping Mart in chat, for him to get a notification. As to not getting an answer, the site's seen a significant uptick in the number of questions posted lately, and a lot of questions are getting overlooked. Asking specifically for assistance, in chat or in a comment as you did, is probably your best bet. $\endgroup$ – hBy2Py Jun 20 '17 at 15:17
  • $\begingroup$ @hBy2Py oh yeah that's good to know. well do you have any advice for finding a solution to my question? it doesn't have to be Mart. $\endgroup$ – bonCodigo Jun 25 '17 at 0:07
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    $\begingroup$ I've been thinking about this on and off -- I can sort of piece together a general approach, but solubility of mixed solutions, especially ~concentrated ones, can get kind of complicated and it's always system-dependent. $\endgroup$ – hBy2Py Jun 25 '17 at 13:02

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