We'd been studying chromatography in our instrumental analysis course and I was wondering if chromatography could induce a conformational change.
For example, you have a straight chain with a hydrophobic side and hydrophilic side; when relaxed, its conformation is such that it has a hydrophilic outer surface and a hydrophobic core.
If you run it through a normal phase column, could the stationary phase cause a change in conformation to, let's say, a hydrophilic core and hydrophobic outer surface?
Also, if this could happen, then it would initially go through the column at a rate similar to the mobile phase and then slow way down, yes? If so, what would be the implication? Would the peaks be broadened?