# Making artificial sweat

I'm currently working on a chemistry investigation wherein I need to dilute a compound with artificial sweat. I have looked at various forums, including this, which gave answers, but is very outdated.

I have also checked on ISOs standards, but it seems that I cannot get the information I want - ISO 105-E04 - without paying a huge sum.

Do any of you know of a recipe for artificial sweat that I can easily conjure in a lab, that's also backed up by various sources?

Thanks

P.S. I cannot just use water + salt, as that would be too simple and cause too many uncertainties for my experiment.

• Sweat is known to contain water, urea, and certain salts like NaCl. Try visiting Wikipedia page about sweat and get the exact composition. – Pritt says Reinstate Monica Apr 19 '17 at 7:28
• ISO 105-E04 describes two solutions, an alkaline solution buffered to pH 8 and an acid solution buffered to pH 5.5. In addition to the phosphate buffer, both solutions contain sodium chloride and L-histidine. Is that what you need? – Loong Apr 19 '17 at 7:31
• @Loong Yes. Unfortunately, it doesn't give the exact proportions of chemicals required to be mixed together unless you pay. In the link I put in my post, someone gave the composition of sweat from another ISO standard, but sadly it was from 2004. – CommandoGeek Apr 19 '17 at 7:40
• @PrittBalagopal Wikipedia offers the composition, but it doesn't state the concentrations of lactic acid or urea. However, it does go in a lot of depth for the less noticeable components, like the different metals. – CommandoGeek Apr 19 '17 at 7:42

The international standard ISO 105-E01:2013 TextilesTests for colour fastness – Part E01: Colour fastness to water includes specifications for two different solutions:

Alkaline solution, freshly prepared, using grade 3 water complying with ISO 3696, containing, per litre:

• 0.5 g of ʟ-histidine monohydrochloride monohydrate ($\ce{C6H9O2N3.HCl.H2O}$);
• 5 g of sodium chloride ($\ce{NaCl}$); and either
• 5 g of disodium hydrogen orthophosphate dodecahydrate ($\ce{Na2HPO4.12H2O}$) or
• 2.5 g of disodium hydrogen orthophosphate dihydrate ($\ce{Na2HPO4.2H2O}$).

The solution is brought to pH 8 (± 0.2) with 0.1 mol/l sodium hydroxide solution.

Acid solution, freshly prepared, using grade 3 water complying with ISO 3696, containing, per litre:

• 0.5 g of ʟ-histidine monohydrochloride monohydrate ($\ce{C6H9O2N3.HCl.H2O}$);
• 5 g of sodium chloride ($\ce{NaCl}$);
• 2.2 g of sodium dihydrogen orthophosphate dihydrate ($\ce{NaH2PO4.2H2O}$).

The solution is brought to pH 5.5 (± 0.2) with 0.1 mol/l sodium hydroxide solution.