I'm trying to do compound optimization for acetic acid on a EI-MS. Later on it would be also run via HPLC to the MS. But I do only get fragments of 53/54 and 32. Also I need a high concentration to be able to do a compound optimization for the 32 fragment (...around 1mM solution).
I looked up other mass spectra of acetic acid online.... and they got nice fragments around 43/45 and 29
...what am I doing wrong?
Edit: for clarification of the question
- We work with ESI, also tried it with APCI
- Acetic acid as a liquid, with mobile phase of water and formic acid
- yes, it is acetic acid
- in the meantime we did a blank (thanks for the suggestion!) and we also got the 32 fragment... but non at 60/61 (for positiv mode) so it should actually work, if we first make Q1 for 61 and then look for fragments
- we re-run a fresh dilution with UV-water ... and got some nicer fragments... but unstable turbo spray signal.