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i am currently working on a project of manufacturing biodiesel from cooking oils by the process "TRANSESTERIFICATION". at the end of this reaction, after the reaction mixture settles I have two layers 1. biodiesel on top 2.glycerol at the bottom. manually the separation can be done by a separating funnel but is there a way by which I could automate the separation process of this mixture?

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From this: http://dx.doi.org/10.1016/S0961-9534(01)00074-5

And this: https://en.m.wikipedia.org/wiki/Glycerol

Apparently most bodiesels have a BP of 340-375 degrees Celsius, while glycerol has a boiling point of 290 degrees Celsius.

I do not know whether or not heating the mixture to 300 degrees would cause a bad reaction or not. But you could try a small scale distillation and find out.

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