# How can I know if there is a phospholipid (phosphatidylcholine) in a solution?

I have a solution which probably contains a phospholipid (phosphatydylcholine), I would like to know if there is an easy method to show the presence of the phospholipid.

I came across TLC, but I couldn't perform it since it's too difficult and expensive to buy chloroform. So, If you guys know an easy method to show the presence of phospholipids please reply.

There are no simple methods available for analysis of phospholipids since the close range of polarity between different phospholipid species makes detection difficult. Oil chemists often use spectroscopy to determine total Phosphorus abundance and then calculate approximate mass of phospholipids based on molecular weight of expected fatty acid species. Modern lipid profiling employs more absolute methods of analysis, with nuclear magnetic resonance spectroscopy (NMR), particularly $\ce{{^{31}P}}$-NMR, while HPLC-ELSD provides relative values.