We've been told our lab report has to be written with "% v/v" instead of volume. I understand how to calculate it, however I'm unsure if I'm doing the right thing when it comes to using it later on. Example:
2ul 'W', 4ul 'X', 2.2ul 'Y', 1.8ul 'Z' is mixed in an eppendorf. It is incubated for 1 hour. After incubation, 0.95ml of 'B' is addded and then 150ul of the final mixture spread on plates.
I know the first bit would be: 20% v/v 'W', 40% v/v 'X', 22% v/v 'Y', 18% v/v 'Z' is mixed in an eppendorf.
But I dont know what to do when it comes to after the incubation?
I'm generally pretty good with this stuff but I've never had to think of it this way. Would, when 0.95ml is added, I do 0.95/0.95+2ul+4ul+2.2ul+1.8ul?