I am currently working on a project and the protocol says the following, "Wash resin 3 times 10 vols of the extract buffer." This is very confusing to me and I am not exactly sure what the 10 vols part means. I tried to look it up, however, the few explanations I found were still confusing. I am new to research, and have not been exposed really to the "lingo".

  • 4
    $\begingroup$ Some experimental procedures are only good enough to be chucked at the heads of the people who wrote them for inclarity — your’s is an example. $\endgroup$
    – Jan
    Oct 30, 2016 at 22:09

1 Answer 1


I think you mean "wash resin 3 times with 10 vols of the extract buffer".

Vol means volume strength. It tells you how much of a certain element in milliliters will be RELEASED from a certain substance. In this case, your buffer is supposed to release 10 vol of something. That's all it means.

However, I am surprised about the usage of vol in this context. Volume strength is usually a term directly associated with hydrogen peroxide, and dealing with the release of a certain volume of oxygen.


Your Answer

By clicking “Post Your Answer”, you agree to our terms of service and acknowledge you have read our privacy policy.

Not the answer you're looking for? Browse other questions tagged or ask your own question.