I recently carried out a Buchwald–Hartwig reaction to attach a morpholin-4-yl group to an aromatic ring:

Reaction scheme

In the proton NMR of the (columned) product, I found the peaks corresponding to the morpholine protons. The multiplet at 3.86 ppm comes from the protons next to oxygen, and the protons next to nitrogen give rise to the multiplet at 3.04 ppm:

enter image description here

enter image description here

The spectrum was obtained on a 400 MHz spectrometer and the values of the "coupling constants" are shown in the second multiplet. (They are identical in the first one.)

Why do the peaks have the fine structure that they do? Based on a simplistic analysis (the "n+1 rule"), I would expect a 1:2:1 triplet for both of these peaks.

In my crude NMR (pre-column) there was some excess morpholine and those peaks had exactly the same fine structure.

Does it have something to do with the chair conformation of the six-membered ring and the differentiation between axial and equatorial protons?

  • 2
    $\begingroup$ I did notice that type of ‘crown’ structure before in NMRs of THF, but I am at a loss what actually causes it … $\endgroup$
    – Jan
    Jul 29 '16 at 22:26
  • $\begingroup$ I once naively gave morpholine as a spectroscopy unknown to students, assuming it would give a nice clean pair of triplets. After the student came to ask about the spectra, they got a new unknown and morpholine went on the "do not assign" list. $\endgroup$ May 20 '18 at 1:20

It looks as if the NMR of morpholine is an AA′XX′ spectrum (the chemical shift difference is 0.80 ppm, or 320 Hz on your spectrometer, two orders of magnitude larger than the coupling constant).

Unlike linear AA′XX′ systems where the bonds can rotate, in the morpholine it very much has a fixed conformation.

enter image description here

In this fixed conformation, the protons are predominantly in a 'gauche' type arrangement due to the shape of the chair, this is where the appearance of the 'triplet' comes from in the NMR spectrum.

The image below is from Hans Reich's NMR notes, and shows some simulated NMR spectra depending on the gauche/anti ratio (Karpus equation), alone with a true spectrum of cyanomorpholine (which you can see looks basically like your spectrum above).

enter image description here

Source: Hans Reich (wisc.edu)

As a complete side-note. In TopSpin (which is what it looks like you used to process your NMR data) you can change the window function to use a Gaussian function, for strong spectra (which it looks like you have), this can help get a bit more resolution (at the expense of S:N- peaks on the baseline will disappear) such that what looks like a broad slightly messy spectrum actually gives resolved multiplets where the J values can be calculated.

In TopSpin, this can be done by entering the wm command; select "gaussian" from the drop-down list, and enter the two required parameters LB and GB (a decent starting choice is -2 and 0.2). The modified FID will now appear; this can be Fourier transformed using ft and automatically phase-corrected using apk to give the modified spectrum. [Even shorter is to sequentially type the commands lb -2, gb 0.2, and gfp into the command line.]

In MestreNova, the apodisation interface may be brought up from the Processing menu (or directly with the hotkey W) and the relevant parameters set in the same fashion.


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