In this paper on page 1985 they say:

Fluorescence analysis confirmed that enhanced protein binding at higher electron dose depends only to a small extent on the slight increase in the lateral dimensions of the nanodeposits but to a much larger degree on their height (Figure 4E)

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The amount of adsorbed IgG-Cy3 is largely independent of the actual area of the nominal 100 nm islands but scales with the height of the nanodeposits.

I don't understand this yet: why do I see that the fluorescence is proportional to area in this figure?


The amount of adsorbed protein is proportional to the area of the 100 nm islands. Figure 4E proves it. The authors misinterpreted their own figure.

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