I have done an untargeted metabolomics study using LC-MS. There is one peak that matches to a biologically relevant compound but analyte eluted at the beginning of the LC run and the peak shape is far from ideal.

Do I assume the peak is an artifact, or can I say that there is a compound with that m/z in my sample? Ideally I would run the experiment again with standards, but I cannot do that due to time constraints. Someone else will follow up on the interesting compounds that I've identified and I wouldn't want to send them in the wrong direction.

Below is the peak in question, and following it is an example of a "good" peak from the same dataset.

Bad peak

Good peak


Your Answer

By clicking “Post Your Answer”, you agree to our terms of service, privacy policy and cookie policy

Browse other questions tagged or ask your own question.