Question on my practice exam. The answers to the questions were not released, otherwise I could work backwards to figure out how to solve it. As it stands, though, I can only guess the method to solve this question.
The extinction coefficients of the 4 natural nucleoside-5'monophosphates @ 260nm (Cavaluzzi,MJ,Nucleic Acids Res., 2004, 32,e13).
*pdA = 1.506 E4 L/mol/cm *pdG = 1.218 E4 -- *pdC = 7.10 E3 -- *pdT = 8.56 E3 --
Find the Extinction Coefficient of a single stranded, 5'-phosphorylated Dickerson Dodecamer sequence d(pCGCGAATTCGC)
If I saw this question on a test, my first instinct would be to add up the values for pdC+pdG+pdC etc...(following the sequence of letters). Or maybe average them all.
We did learn the Beer-Lambert Law used in absorption spectrum, where e was extinction coeffecient: $$A=E(extinction)*b*c$$ but I can see no way to use this equation, since i'm only given a wavelength and a whole bunch of coefficients.
Does anyone know how to solve this? Is there an equation I'm missing?
Thanks in advance.