What is the percent concentration of hydrogen peroxide which has a concentration of $500~\mu\mathrm{M}$?

Is $500~\mu\mathrm{M}$ $\ce{H2O2}$ toxic to the eyes?

A particular honey has a concentration of $\ce{H2O2}$ of $500~\mu\mathrm{M}$ after a 24-hour incubation time, after it has been diluted. I am trying to figure out what the concentration is in percent (%).

I recalculated and the result is actually $0.00153\%$ for $500~\mu\mathrm{M}$. $0.0012\%$ is the equivalent to $400~\mu\mathrm{M}$, but I need someone to confirm this. Someone on researchgate wrote that $30\%$ of $\ce{H2O2}$ is $9.8~\mathrm{M}$.

  • $\begingroup$ Note that in the first line you have given the concentration of hydrogen peroxide, not the molar mass. $\endgroup$ Aug 27, 2015 at 5:09

2 Answers 2


I will assume you are asking for percent mass/volume, $\mathrm{g/ml}$, since to calculate percent mass/mass you need the density of the liquid (unless you assume it is water which is $1~\mathrm{g/ml}$ so will give the same answer).

We have $500~\mu\mathrm{M}$ of hydrogen peroxide, which means we have $500~\mu\mathrm{mol}$ of hydrogen peroxide per 1 litre of solution. $500~\mu\mathrm{mol} = 0.0005~\mathrm{mol}$. Now you can work out the molar mass of hydrogen peroxide and multiply it by the number of moles to get the mass of hydrogen peroxide per litre of solution. This gives $0.017~\mathrm{g}$. There are $1000~\mathrm{ml}$ in 1 litre. So you have $0.017~\mathrm{g}/1000~\mathrm{ml} = 0.000017 = 0.0017\%$.

For your question about whether hydrogen peroxide is toxic to the eyes, you can search the internet for the Materials Safety Data Sheet (MSDS) of hydrogen peroxide (Sigma-Aldrich is a good site to search). This contains all the hazards associated with this chemical, and when you do laboratory work you always have to look these up for each chemical before you start a reaction. The MSDS states that hydrogen peroxide can cause severe eye damage, among other hazards.

  • $\begingroup$ Thank you for your answer it seem like I wasn't far off. I do not know how H2O2 in honey is measured but I assume it's per litre. So it's about a 1000-fold less than standard disinfectants. $\endgroup$ Aug 26, 2015 at 13:04
  • $\begingroup$ I have updated your post with chemistry markup. If you want to know more, please have a look here and here. Please do not use markup in the title field, see here for details. There is also a second question included by the OP, I am vey happy to upvote this answer if you could include a little statement about that. $\endgroup$ Aug 27, 2015 at 5:10
  • $\begingroup$ thanks, I'll try to next time. I've included an answer for the other question $\endgroup$
    – k--
    Aug 27, 2015 at 8:55

Regarding toxicity: Honey solutions are actually sometimes used in ophthalmology. There is a company called Optimel that sells 16% manuka honey eye drops OTC for treating dry eye syndrome but note that very little or almost nothing H2O2 is released in manuka honey. Another example is this case report in which they used a 25% w/v silver fir honey solution as eye drops for the treatment of a corneal ulcer with success.

I found an article in which the use of H2O2 in the eyes in various concentrations is outlined. At the concentration below 60 ppm (the lowest safe point) the eye’s natural enzyme mechanisms can break down the small amount of H2O2 making it safe.

So a 25% w/v (silver fir) honey solution contains around 0.00085% or 8.5 ppm of H2O2 which would make it safe for the use in eyes.


Your Answer

By clicking “Post Your Answer”, you agree to our terms of service, privacy policy and cookie policy

Not the answer you're looking for? Browse other questions tagged or ask your own question.