In UV spectroscopy, when a beam of light is shone into solution, what is scattering loss during this process?


1 Answer 1


Scattering is the redirection of the 'beam' of light, as is illustrated in the diagram below:

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Source: University of Wisconsin

The description from the document Fundamentals of modern UV-visible spectroscopy (Figure 46) states that

Scattering causes an apparent absorbance because less light reaches the detector

They also detail two main types of scattering in UV spectroscopy (Figure 47):

Rayleigh scattering: Particles small relative to wavelength
Tyndall scattering: Particles large relative to wavelength


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