# Measuring the % of pectin in a fibre after enzymatic treatment with pectinase

I will remove the pectin from hemp fibres with Bioprep 3000L pectinase. How can I measure the % of pectin before and after for loose fibres? I only found a way to measure it for fabric, but I will perform this test on just loose hemp fibres.

• The meter for measurement for fabrics is called: ruthenium red dye method. Apr 21 '15 at 7:39

In Crystal-Structure of Ruthenium Red and Stereochemistry of its Pectic Stain by Clarence Sterling, Am. J. Bot. 1970, 57, 172-175, the specific binding of Ruthenium Red (RR, CAS-RN: 11103-72-3) along a pectin chain, that is, between a $\ce{COOH}$ of one galacturonic acid unit and the $\ce{OH}$ of the next, has been described.
In a more recent publication, Qualitative distinction of carboxyl group distributions in pectins with ruthenium red, published in Bot. Bull. Acad. Sin., 1999, 40, 115-119 (PDF), the method was applied to orange peel pectines of different origin. Here, the dye-pectin adducts were precipitated by addition of $\ce{CaCl2}$ and centrifugated. Then, the absorbtion of the supernatant solution and a stock solution of the dye at $\lambda = 534\,\mathrm{nm}$ were compared.