# How to detect azide group on a primer?

I bought azide-labeled primer for click chemistry, but it didn't work. So I want to prove the existence of azide group. How could I achieve this? I found a method to add PPh3 to Kaiser test reagents, but the concentration of the primer is too low (the protocol needs the solution be higher than 0.1M) and I cannot see the reaction.

• Does click reaction work with labeled nucleotide? Dec 4 '14 at 5:21
• No. That's why I would suspect there's problem with azide group :( Jan 30 '15 at 15:15