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for my enzyme fluorescent assay measurements in soil samples I have to calculate my standard curves. In a protocol, I have found this table where I cannot comprehend the reasoning how the author has gotten to the numbers in the column "Concentration (in nM)".

As a recap: There is already 200µl of buffer in the microplate well, then step by step a stock solution of 10µM or 50µM is added as presented in the table. (so in sum a volume of 250µl in every well)

What is the calculation reasoning behind the column "Concentration (in nM)"?

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  • $\begingroup$ Add your attempts at calculating the concentrations. What is the dilution formula? What is the relationship between micro- and nano- prefixes? $\endgroup$
    – ACR
    Commented Aug 9 at 20:55

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