For a biological experiment I have acquired $\pu{10 mg}$ of CHIR 99021 (laduviglusib). In order to obtain a $\pu{10 mM}$ stock solution, I had to dissolve the powder in $\pu{2.15 mL}$ of dimethyl sulfoxide (DMSO). Unfortunately, I tried to dissolve the powder in distilled water. That powder is said to be insoluble in water. In order to obtain again the powder, I tried to centrifuge the solution $(\pu{1500 rpm}, \ \pu{20 min})$, hoping that the powder would precipitate (sedimented), but nothing happened.

I would like to know how to dissolve that solution: I am thinking to put another $\pu{2.15 mL}$ of DMSO in the water solution in order to have a stock solution of $\pu{5 mM}$ (which would be fine for me).

What would you suggest? Thank you very much.

  • $\begingroup$ please do not capitalized the chemical names unless they are in the front of the sentences. $\endgroup$ Commented May 18 at 23:50
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    $\begingroup$ Since you are in a bioanalytical lab (I assumed), you can freeze-dried the sample to evaporate water before adding $\pu{2.15 mL}$ DMSO to the powder (hope vacuum didn't take away the fine powder). $\endgroup$ Commented May 18 at 23:55
  • $\begingroup$ @MathewMahindaratne Thank you very much for your kind reply. Would you suggest adding DMSO in water, obtaining a more diluted solution? Would it work in that way, from a chemical point of wiew? Thank you! $\endgroup$
    – felbamato
    Commented May 20 at 9:32
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    $\begingroup$ Laduviglusib might dissolve in 1:1 DMSO/water. But there is a risk it might not because this is the reverse of usual procedure. Normally, we dissolve drugs in DMSO and then add water to dilute it. $\endgroup$ Commented May 20 at 15:00


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