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I stained my work station with Coomassie blue solution during my SDS-PAGE protocol. I have been cleaning but the solution seems to can't be removed. What should I use to remove all these stains?

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  • $\begingroup$ What is the surface of the work-station made of? How long have the stains been there? What are you using to clean the stains? $\endgroup$
    – ananta
    May 31, 2023 at 10:19
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    $\begingroup$ What is a SDS-PAGE protocol ? $\endgroup$
    – Maurice
    May 31, 2023 at 12:07
  • $\begingroup$ Coomassie Blue is colorless at pH 1 - 3. So by adding a $\ce{HCl}$ solution diluted to about 0.01 M, it should make the blue dye colorless. $\endgroup$
    – Maurice
    May 31, 2023 at 12:14
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    $\begingroup$ The color will come back once HCl evaporates. A very dilute solution of bleach (used as per manufacturer's and lab instructions) should remove dye stains. $\endgroup$
    – AChem
    May 31, 2023 at 12:34
  • $\begingroup$ Use organic solvents, like ethanol, DMSO, etc. $\endgroup$
    – ananta
    May 31, 2023 at 14:23

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