I stained my work station with Coomassie blue solution during my SDS-PAGE protocol. I have been cleaning but the solution seems to can't be removed. What should I use to remove all these stains?
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$\begingroup$ What is the surface of the work-station made of? How long have the stains been there? What are you using to clean the stains? $\endgroup$– anantaMay 31 at 10:19
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1$\begingroup$ What is a SDS-PAGE protocol ? $\endgroup$– MauriceMay 31 at 12:07
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$\begingroup$ Coomassie Blue is colorless at pH 1 - 3. So by adding a $\ce{HCl}$ solution diluted to about 0.01 M, it should make the blue dye colorless. $\endgroup$– MauriceMay 31 at 12:14
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4$\begingroup$ The color will come back once HCl evaporates. A very dilute solution of bleach (used as per manufacturer's and lab instructions) should remove dye stains. $\endgroup$– AChemMay 31 at 12:34
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$\begingroup$ Use organic solvents, like ethanol, DMSO, etc. $\endgroup$– anantaMay 31 at 14:23
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