I want to prepare 10mL of 100mg/ml stock solution from plant extract using water. However, the plant extract I’m using have solubility problem, it can’t fully dissolved in water. So I need DMSO to dissolve it. The final concentration of DMSO has to be 1% in 100mg/ml of the plant extract.
Is this the correct way to do it ?
- Add 1000mg of plant extract powder into 10mL of DMSO (100%) as stock solution
- Take 0.1mL from stock solution and add into 9.9mL of water to get 1% DMSO + water of 100mg/ml plant extract (My working solution)
After that, I want to further get 50mg/ml and 25mg/ml from my working solution using dilution method. My question is,can I assume the concentration from 0.1mL stock solution be 100mg/ml ? And after dilution, the dmso percentage would remained the same or nah ? I got confused with this and if I’m wrong, please help correct me. Thanks in advance 😩