I have about 20-30 grams of purified mammalian DNA preserved in EDTA. It is clumped up and solid. I need to dissolve and homogenize it, so it can be aliquoted, without damaging or lysing or fragmenting the DNA. What is the best way to do this?

  • $\begingroup$ I think you can do this with TE buffer or water, the latter of which needs to be 18 M$\Omega$ RO. $\endgroup$ Commented Dec 1, 2021 at 17:13
  • $\begingroup$ How large are the DNA pieces (eg number of base pairs)? That’s a big factor in how susceptible they are to mechanical damage. If it’s a small plasmid, you can pipette vigorously without much worry, but if it’s genomic DNA you need to be much gentler $\endgroup$
    – Andrew
    Commented Dec 2, 2021 at 1:51
  • $\begingroup$ @Andrew I updated the questions. It's mammalian genomic DNA. $\endgroup$
    – Shaka Boom
    Commented Dec 2, 2021 at 12:41

1 Answer 1


I suspect you'll get a much better answer if you migrate this question to biology SE, but here's what I've done:

  1. add your chosen volume of nuclease-free TE buffer or water (assuming the DNA is precipitated with salts).

  2. Leave it in the fridge (~4 C) overnight to allow for slow hydration.

  3. Gently resuspend the DNA by very slowly pipetting the solution up and down using a 1 mL pipette with about 1 cm of the tip cut off so that the opening is substantially larger than normal.

  4. Make aliquots for future use so as to minimize handling.


Your Answer

By clicking “Post Your Answer”, you agree to our terms of service and acknowledge you have read our privacy policy.

Not the answer you're looking for? Browse other questions tagged or ask your own question.