I have about 20-30 grams of purified mammalian DNA preserved in EDTA. It is clumped up and solid. I need to dissolve and homogenize it, so it can be aliquoted, without damaging or lysing or fragmenting the DNA. What is the best way to do this?
I suspect you'll get a much better answer if you migrate this question to biology SE, but here's what I've done:
add your chosen volume of nuclease-free TE buffer or water (assuming the DNA is precipitated with salts).
Leave it in the fridge (~4 C) overnight to allow for slow hydration.
Gently resuspend the DNA by very slowly pipetting the solution up and down using a 1 mL pipette with about 1 cm of the tip cut off so that the opening is substantially larger than normal.
Make aliquots for future use so as to minimize handling.