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It seems like the idea of Ion Exchange Chromatography is to get a maximum amount of exposure of your gel to your solution. It seems like overhead stirring and a gel bed would be more likely to be successful here.

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    $\begingroup$ It seems like you are focussing on the ion exchange part and ignoring the chromatography. $\endgroup$
    – Ed V
    Commented Nov 18, 2021 at 23:53
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    $\begingroup$ Yes, with a solid ion-exchange bead sitting in solution, you are doing an equilibrium experiment. You do not have a true equilibrium condition in a chromatography column. $\endgroup$
    – ACR
    Commented Nov 18, 2021 at 23:55
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    $\begingroup$ Chromatography is a dynamic separation technique, done on columns or planes. Usage of ion-exchange resin is just circumstance. There is no separation of analytes or substances in the static gel bed scenario, just different distribution constants. $\endgroup$
    – Poutnik
    Commented Nov 19, 2021 at 6:21
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    $\begingroup$ Do you bath you car's engine with gas, or do you prefer, for practical reasons, to put the gas station's nozzle to this funnel shaped entry to channel the gas into a the tank? (irony!) $\endgroup$
    – Buttonwood
    Commented Nov 19, 2021 at 7:45

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