I'm troubleshooting an IHC staining issue, and according to Thermo-Fischer's website, "Deionized water can sometimes contain peroxidase inhibitors that can significantly impair enzyme activity." What exactly could deionized water contain that would inhibit peroxidase?

  • $\begingroup$ Would be nice to have a link. Found this: thermofisher.com/se/en/home/life-science/protein-biology/… $\endgroup$
    – Buck Thorn
    Feb 22 '21 at 18:51
  • $\begingroup$ I'll hazard a guess: minute amounts of heavy metals maybe? Oxygen (although that sounds farfetched)? $\endgroup$
    – Buck Thorn
    Feb 22 '21 at 20:20
  • $\begingroup$ @BuckThorn, I think it must be leachates from the ion exchange resins and perhaps bacterial contamination. I don't think deionized water has traces of metals. $\endgroup$
    – M. Farooq
    Feb 23 '21 at 5:03
  • $\begingroup$ @M.Farooq Yes, good point. The presence of metals would violate the designation DI. I'll leave my comment up in case someone else has this odd thought. $\endgroup$
    – Buck Thorn
    Feb 23 '21 at 7:26

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