I have completed a whole set of FITR-ATR spectra analysing 6 solutions of ethanol-water mixtures, all at different concentrations of each.
I have now been instructed to use the method of differential absorption to determine the concentration of ethanol. I am subtracting the absorbance at the base of the C-H stretch of ethanol from the maximum absorbance of the same stretch so now I have a difference in absorbance value. At this stage, I am a little confused as to how I use this value to obtain the concentration of ethanol. All I know is that the Beer-Lambert law is involved somehow.
Any help with this would be appreciated. Thanks.