I have 100 micrograms of STAT1 and its molecular weight is 90 kDa. I calculated the number of moles to be 1 nmoles. The specific activity of JAK1 (the kinase) is 110 nmoles of phosphate per minute per mg of total protein at 30°C and the activity was determined at a final concentration of 0.83 micrograms/milliliters. What amout of JAK1 do I need?
I tried many ways but I don't get how to link the data.